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FEM1C Polyclonal antibody

FEM1C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14037-1-AP

Print datasheet

Synonyms

Protein fem-1 homolog C, FEM1-gamma, FEM1A, FEM1 gamma, FEM1

验证数据展示

  • WB analysis using 14037-1-AP

    WB analysis using 14037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14037-1-AP

    IF Staining of HeLa using 14037-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FEM1C antibody (14037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human heart using 14037-1-AP

    IHC staining of human heart using 14037-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, HepG2 cells, rat brain tissue
Positive IHC detected inhuman heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14037-1-AP targets FEM1C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FEM1C fusion protein Ag5099 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fem-1 homolog c (C. elegans)
Synonyms Protein fem-1 homolog C, FEM1-gamma, FEM1A, FEM1 gamma, FEM1
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC028369
Gene Symbol FEM1C
Gene ID (NCBI) 56929
RRIDAB_2878002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.

实验方案

Product Specific Protocols
WB protocol for FEM1C antibody 14037-1-APDownload protocol
IHC protocol for FEM1C antibody 14037-1-APDownload protocol
IF protocol for FEM1C antibody 14037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols