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FER Polyclonal antibody, PBS Only

FER Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25287-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of NIH/3T3 using 25287-1-AP (same clone as 25287-1-PBS)

    WB analysis of NIH/3T3 using 25287-1-AP (same clone as 25287-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

    IP experiment of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

    IP result of anti-FER (IP:25287-1-AP, 4ug; Detection:25287-1-AP 1:1000) with MCF-7 cells lysate 2400ug. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 25287-1-AP (same clone as 25287-1-PBS)

    IHC staining of human kidney using 25287-1-AP (same clone as 25287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FER antibody included
  • IHC staining of human kidney using 25287-1-AP (same clone as 25287-1-PBS)

    IHC staining of human kidney using 25287-1-AP (same clone as 25287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FER antibody included
  • IF Staining of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

    IF Staining of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 25287-1-AP (FER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FER Ready-To-Use IHC Kit. FER primary antibody included.

产品信息

25287-1-PBS targets FER in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen FER fusion protein Ag18173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fer (fps/fes related) tyrosine kinase
Synonyms
Calculated Molecular Weight 822 aa, 95 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC017060
Gene Symbol FER
Gene ID (NCBI) 2241
RRIDAB_2880009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16591
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.