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FES Polyclonal antibody

FES Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28194-1-AP

Print datasheet

Synonyms

feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps

验证数据展示

  • IHC staining of human colon cancer using 28194-1-AP

    IHC staining of human colon cancer using 28194-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 28194-1-AP

    IHC staining of human colon cancer using 28194-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 28194-1-AP

    WB analysis using 28194-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28194-1-AP (FES antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHL-60 cells, THP-1 cells, U-937 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28194-1-AP targets FES in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen FES fusion protein Ag28095 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name feline sarcoma oncogene
Synonyms feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps
Calculated Molecular Weight 822 aa, 94 kDa
Observed Molecular Weight 93 kDa, 79 kDa, 87 kDa, 85 kDa
GenBank Accession NumberBC035357
Gene Symbol FES
Gene ID (NCBI) 2242
RRIDAB_2881086
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07332
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Feline sarcoma oncogene protein (FES) is a member of the FES/FES-related (FER) subfamily of non-receptor protein tyrosine kinases. FES was originally identified a retroviral oncogene in avian and feline retroviruses. Articles reported that FES plays a role in growth and differentiation of myeloid hematopoietic cells, vascular endothelial cells and neurons. And it also involved in the regulation of cytoskeletal rearrangement (PMID:15929003). Addition, a recent study suggests that FES may function both tumorigenic and tumor-suppressive effects (PMID: 28952025).

实验方案

Product Specific Protocols
WB protocol for FES antibody 28194-1-APDownload protocol
IHC protocol for FES antibody 28194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols