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验证数据展示

  • IHC staining of human colon cancer using 28194-1-AP

    IHC staining of human colon cancer using 28194-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 28194-1-AP

    IHC staining of human colon cancer using 28194-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 28194-1-AP

    WB analysis using 28194-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28194-1-AP (FES antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

FES Polyclonal antibody

FES Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28194-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, THP-1 cells, U-937 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28194-1-AP targets FES in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 FES fusion protein Ag28095 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 feline sarcoma oncogene
别名 feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps
计算分子量 822 aa, 94 kDa
观测分子量 93 kDa, 79 kDa, 87 kDa, 85 kDa
GenBank蛋白编号BC035357
基因名称 FES
Gene ID (NCBI) 2242
RRIDAB_2881086
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07332
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Feline sarcoma oncogene protein (FES) is a member of the FES/FES-related (FER) subfamily of non-receptor protein tyrosine kinases. FES was originally identified a retroviral oncogene in avian and feline retroviruses. Articles reported that FES plays a role in growth and differentiation of myeloid hematopoietic cells, vascular endothelial cells and neurons. And it also involved in the regulation of cytoskeletal rearrangement (PMID:15929003). Addition, a recent study suggests that FES may function both tumorigenic and tumor-suppressive effects (PMID: 28952025).

实验方案

Product Specific Protocols
WB protocol for FES antibody 28194-1-APDownload protocol
IHC protocol for FES antibody 28194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols