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FEV Polyclonal antibody

FEV Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 25058-1-AP

Print datasheet

Synonyms

FEV, FEV (ETS oncogene family), Fifth Ewing variant protein, HSRNAFEV, PC12 ETS factor 1, PET 1, PET1, Protein FEV

验证数据展示

  • WB analysis of fetal human brain using 25058-1-AP

    WB analysis of fetal human brain using 25058-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25058-1-AP (FEV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 25058-1-AP

    WB analysis of mouse brain using 25058-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25058-1-AP (FEV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected infetal human brain tissue, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

25058-1-AP targets FEV in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen FEV fusion protein Ag20647 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FEV (ETS oncogene family)
Synonyms FEV, FEV (ETS oncogene family), Fifth Ewing variant protein, HSRNAFEV, PC12 ETS factor 1, PET 1, PET1, Protein FEV
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC023511
Gene Symbol FEV
Gene ID (NCBI) 54738
RRIDAB_2879877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99581
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FEV belongs to the ETS transcription factor family. ETS family members have a highly conserved 85-amino acid ETS domain that binds purine-rich DNA sequences. The alanine-rich C-terminus of this gene indicates that it may act as a transcription repressor. This gene is exclusively expressed in neurons of the central serotonin (5-HT) system, a system implicated in the pathogeny of such psychiatric diseases as depression, anxiety, and eating disorders. In some types of Ewing tumors, this gene is fused to the Ewing sarcoma (EWS) gene following chromosome translocations.

实验方案

Product Specific Protocols
WB protocol for FEV antibody 25058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article