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FEZ1 Polyclonal antibody

FEZ1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12100-1-AP

Print datasheet

Synonyms

FEZ1, Zygin 1, Zygin I

验证数据展示

  • IHC staining of human gliomas using 12100-1-AP

    IHC staining of human gliomas using 12100-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12100-1-AP (FEZ1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse brain using 12100-1-AP

    WB analysis of mouse brain using 12100-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12100-1-AP (FEZ1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12100-1-AP

    IP experiment of mouse brain using 12100-1-AP

    IP result of anti-FEZ1 (IP:12100-1-AP, 4ug; Detection:12100-1-AP 1:300) with mouse brain tissue lysate 3600ug.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12100-1-AP targets FEZ1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FEZ1 fusion protein Ag2744 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fasciculation and elongation protein zeta 1 (zygin I)
Synonyms FEZ1, Zygin 1, Zygin I
Calculated Molecular Weight 392 aa, 45 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC009545
Gene Symbol FEZ1
Gene ID (NCBI) 9638
RRIDAB_2877825
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99689
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FEZ1 antibody 12100-1-APDownload protocol
IHC protocol for FEZ1 antibody 12100-1-APDownload protocol
IP protocol for FEZ1 antibody 12100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Front Neurosci

Expression of the schizophrenia associated gene FEZ1 in the early developing fetal human forebrain

Authors - Maznah Alhesain
View Article
humanIHC

Breast Cancer (Dove Med Press)

Identification and Validation of Novel Metastasis-Related Immune Gene Signature in Breast Cancer

Authors - Shen Ma
View Article