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验证数据展示

  • WB analysis using 13141-1-AP

    WB analysis using 13141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 13141-1-AP

    WB analysis using 13141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of SK-N-SH using 13141-1-AP

    IP experiment of SK-N-SH using 13141-1-AP

    IP result of anti-FGD1 (IP:13141-1-AP, 4ug; Detection:13141-1-AP 1:600) with SK-N-SH cells lysate 1160 ug.

  • IHC staining of mouse kidney using 13141-1-AP

    IHC staining of mouse kidney using 13141-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13141-1-AP (FGD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 13141-1-AP

    IF Staining of U2OS using 13141-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using FGD1 antibody (13141-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

FGD1 Polyclonal antibody

FGD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13141-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, SK-N-SH cells, U2OS cells
Positive IP detected inSK-N-SH cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13141-1-AP targets FGD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FGD1 fusion protein Ag3861 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FYVE, RhoGEF and PH domain containing 1
Synonyms Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY
Calculated Molecular Weight 901 aa, 91 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC034530
Gene Symbol FGD1
Gene ID (NCBI) 2245
RRIDAB_3085409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98174
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).

实验方案

Product Specific Protocols
WB protocol for FGD1 antibody 13141-1-APDownload protocol
IHC protocol for FGD1 antibody 13141-1-APDownload protocol
IF protocol for FGD1 antibody 13141-1-APDownload protocol
IP protocol for FGD1 antibody 13141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols