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验证数据展示

  • WB analysis of Raji using 27068-1-AP

    WB analysis of Raji using 27068-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 27068-1-AP (FGD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27068-1-AP

    IHC staining of human tonsillitis using 27068-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FGD2 antibody included
  • IHC staining of human tonsillitis using 27068-1-AP

    IHC staining of human tonsillitis using 27068-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FGD2 antibody included

FGD2 Polyclonal antibody

FGD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27068-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Zinc finger FYVE domain-containing protein 4, ZFYVE4, FYVE, RhoGEF and PH domain-containing protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FGD2 Ready-To-Use IHC Kit. FGD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27068-1-AP targets FGD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 FGD2 fusion protein Ag25782 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FYVE, RhoGEF and PH domain containing 2
别名 Zinc finger FYVE domain-containing protein 4, ZFYVE4, FYVE, RhoGEF and PH domain-containing protein 2
计算分子量 655 aa, 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC023645
基因名称 FGD2
Gene ID (NCBI) 221472
RRIDAB_2880740
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z6J4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGD2 is a member of the formin family of proteins, which are known for their role in regulating the assembly of actin filaments. Formins are characterized by their ability to nucleate and elongate actin filaments, and they play a crucial role in processes that require dynamic actin structures, such as cell motility and cell division. Mutations or alterations in FGD2 have been associated with certain developmental disorders and diseases. For example, mutations in FGD2 are a cause of faciogenital dysplasia (Aarskog-Scott syndrome), a condition characterized by facial and genital abnormalities and short stature.

实验方案

Product Specific Protocols
WB protocol for FGD2 antibody 27068-1-APDownload protocol
IHC protocol for FGD2 antibody 27068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols