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FGD2 Polyclonal antibody

FGD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27068-1-AP

Print datasheet

Synonyms

Zinc finger FYVE domain-containing protein 4, ZFYVE4, FYVE, RhoGEF and PH domain-containing protein 2

验证数据展示

  • WB analysis of Raji using 27068-1-AP

    WB analysis of Raji using 27068-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 27068-1-AP (FGD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27068-1-AP

    IHC staining of human tonsillitis using 27068-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27068-1-AP

    IHC staining of human tonsillitis using 27068-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27068-1-AP targets FGD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FGD2 fusion protein Ag25782 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FYVE, RhoGEF and PH domain containing 2
Synonyms Zinc finger FYVE domain-containing protein 4, ZFYVE4, FYVE, RhoGEF and PH domain-containing protein 2
Calculated Molecular Weight 655 aa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC023645
Gene Symbol FGD2
Gene ID (NCBI) 221472
RRIDAB_2880740
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6J4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGD2 is a member of the formin family of proteins, which are known for their role in regulating the assembly of actin filaments. Formins are characterized by their ability to nucleate and elongate actin filaments, and they play a crucial role in processes that require dynamic actin structures, such as cell motility and cell division. Mutations or alterations in FGD2 have been associated with certain developmental disorders and diseases. For example, mutations in FGD2 are a cause of faciogenital dysplasia (Aarskog-Scott syndrome), a condition characterized by facial and genital abnormalities and short stature.

实验方案

Product Specific Protocols
WB protocol for FGD2 antibody 27068-1-APDownload protocol
IHC protocol for FGD2 antibody 27068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols