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验证数据展示

  • WB analysis using 20347-1-AP

    WB analysis using 20347-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20347-1-AP

    IHC staining of human placenta using 20347-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20347-1-AP (FGD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FGD3 antibody included
  • IHC staining of human placenta using 20347-1-AP

    IHC staining of human placenta using 20347-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20347-1-AP (FGD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FGD3 antibody included
  • IF Staining of A549 using 20347-1-AP

    IF Staining of A549 using 20347-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 20347-1-AP (FGD3 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

FGD3 Polyclonal antibody

FGD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20347-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Zinc finger FYVE domain-containing protein 5, ZFYVE5, FYVE, RhoGEF and PH domain-containing protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, K-562 cells, HEK-293T cells, HeLa cells, A549 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy FGD3 Ready-To-Use IHC Kit. FGD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20347-1-AP targets FGD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 FGD3 fusion protein Ag14155 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FYVE, RhoGEF and PH domain containing 3
别名 Zinc finger FYVE domain-containing protein 5, ZFYVE5, FYVE, RhoGEF and PH domain-containing protein 3
计算分子量 725 aa, 79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC032429
基因名称 FGD3
Gene ID (NCBI) 89846
RRIDAB_10693616
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JSP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FGD3 antibody 20347-1-APDownload protocol
IHC protocol for FGD3 antibody 20347-1-APDownload protocol
IF protocol for FGD3 antibody 20347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Oncogene

FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression.

Authors - Feng Guo
  • KD Validated
View Article