验证数据展示
经过测试的应用
| Positive IHC detected in | human lung tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20910-1-AP targets FGD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | FGD5 fusion protein Ag15037 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FYVE, RhoGEF and PH domain containing 5 |
| 别名 | FGD5, ZFYVE23 |
| 计算分子量 | 1462 aa, 160 kDa |
| GenBank蛋白编号 | BC035364 |
| 基因名称 | FGD5 |
| Gene ID (NCBI) | 152273 |
| RRID | AB_10733757 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZNL6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FGD5 (or ZFYVE23) is an endothelial specific gene, plays a crucial role in vascular pruning. Deficient expression of FGD5 markedly inhibits neovascularization, indicated by in vitro tube-formation, aortic-ring, and coated-bead assays and by in vivo coated-bead plug assays and studies in the murine retina model. FGD5-deficient endothelial cells fail to migrate. Moreover, the cells are susceptible to proapoptotic stimuli associated with VEGF-stimulated activation of Akt. FGD5 promotes apoptosis-induced vaso-obliteration via induction of the hey1-p53 pathway by direct binding and activation of CDC42.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FGD5 antibody 20910-1-AP | Download protocol |
| IF protocol for FGD5 antibody 20910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep VE-PTP inhibition stabilizes endothelial junctions by activating FGD5.
| ||
Arterioscler Thromb Vasc Biol Facio-genital dysplasia-5 regulates matrix adhesion and survival of human endothelial cells. | ||
Arterioscler Thromb Vasc Biol FGD5 Regulates VEGF Receptor-2 Coupling to PI3 Kinase and Receptor Recycling.
| ||
Dev Cell FGD5 in basal cells induces CXCL14 secretion that initiates a feedback loop to promote murine mammary epithelial growth and differentiation |
