FGF-2 Polyclonal antibody

FGF-2 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11234-1-AP

Print datasheet

Synonyms

Basic fibroblast growth factor, BFGF, FGF2, FGF-2, FGFB, HBGF 2



经过测试的应用

Positive WB detected inHEK-293T cells, SKOV-3 cells, HepG2 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue, mouse lung tissue, rat brain tissue, rat kidney tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy FGF2 Ready-To-Use IHC Kit. FGF2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11234-1-AP targets FGF-2 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibroblast growth factor 2 (basic)
Synonyms Basic fibroblast growth factor, BFGF, FGF2, FGF-2, FGFB, HBGF 2
Calculated Molecular Weight 21 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberNM_002006
Gene Symbol FGF2
Gene ID (NCBI) 2247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09038
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

实验方案

Product Specific Protocols
WB protocol for FGF-2 antibody 11234-1-APDownload protocol
IHC protocol for FGF-2 antibody 11234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nat Nanotechnol

Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.

Authors - Xuefei Lu
WB

Biomaterials

Lung endothelial cell-targeted peptide-guided bFGF promotes the regeneration after radiation induced lung injury.

Authors - Dongwei Guan
humanWB

BMC Cancer

Multi-omics sequencing revealed endostar combined with cisplatin treated non small cell lung cancer via anti-angiogenesis

Authors - Yufei Wang
humanWB,IHC

Wound Repair Regen

Negative pressure wound therapy is associated with upregulation of bFGF and ERK1/2 in human diabetic foot wounds.

Authors - Shao-Ling Yang
humanWB, IHC

Exp Ther Med

Expression of signal transducer and activator of transcription 3 and its phosphorylated form is significantly upregulated in patients with papillary thyroid cancer.

Authors - L I Yan