验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, SKOV-3 cells, HepG2 cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue, mouse lung tissue, rat brain tissue, rat kidney tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | K-562 cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy FGF2 Ready-To-Use IHC Kit. FGF2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 5 publications below |
产品信息
11234-1-AP targets FGF-2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibroblast growth factor 2 (basic) |
| 别名 | FGF2, fibroblast growth factor 2, FGFB, FGF basic, FGF B |
| 计算分子量 | 21 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | NM_002006 |
| 基因名称 | FGF2 |
| Gene ID (NCBI) | 2247 |
| RRID | AB_2941976 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09038 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FGF-2 antibody 11234-1-AP | Download protocol |
| IHC protocol for FGF-2 antibody 11234-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Nanotechnol Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades. | ||
Biomaterials Lung endothelial cell-targeted peptide-guided bFGF promotes the regeneration after radiation induced lung injury. | ||
Diabetes N 6-Methyladenosine demethylase FTO controls macrophage homeostasis in diabetic vasculopathy | ||
BMC Cancer Multi-omics sequencing revealed endostar combined with cisplatin treated non small cell lung cancer via anti-angiogenesis | ||
Toxicol Appl Pharmacol Synergistic anticancer effects of ginsenoside CK and gefitinib against gefitinib-resistant NSCLC by regulating the balance of angiogenic factors through HIF-1α/VEGF | ||
Wound Repair Regen Negative pressure wound therapy is associated with upregulation of bFGF and ERK1/2 in human diabetic foot wounds. |
