• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FGF9 Polyclonal antibody

FGF9 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26554-1-AP

Print datasheet

Synonyms

FGF-9, FGF 9, Heparin-binding growth factor 9, HBGF-9, Glia-activating factor

验证数据展示

  • IHC staining of human stomach using 26554-1-AP

    IHC staining of human stomach using 26554-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26554-1-AP (FGF9 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 26554-1-AP

    IHC staining of human stomach using 26554-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26554-1-AP (FGF9 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of MCF-7 using 26554-1-AP

    IF Staining of MCF-7 using 26554-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FGF9 antibody (26554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IHC detected inhuman stomach tissue, human prostate cancer tissue, human colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26554-1-AP targets FGF9 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen FGF9 fusion protein Ag24244 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibroblast growth factor 9 (glia-activating factor)
Synonyms FGF-9, FGF 9, Heparin-binding growth factor 9, HBGF-9, Glia-activating factor
Calculated Molecular Weight 208 aa, 23 kDa
GenBank Accession NumberBC069692
Gene Symbol FGF9
Gene ID (NCBI) 2254
RRIDAB_2880550
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor 9 (FGF9) is a critically important and elegantly regulated growth factor that was first identified during a screen for factors acting on cells of the central nervous system (CNS). Over the subsequent two decades of research, FGF9 has been revealed to play a significant role in various biological processes. A key feature of its control is reciprocal compartmentalization, particularly during development, with the epithelium serving as a dominant source and mesenchyme as a prime target. This mesenchyme selectivity is achieved through the high affinity of FGF9 to the IIIc isoforms of FGFR1, 2, and 3. FGF9 is widely expressed in the embryo, including in the developing heart and lungs, and more selectively in adult tissues, such as the CNS and kidneys.

实验方案

Product Specific Protocols
IHC protocol for FGF9 antibody 26554-1-APDownload protocol
IF protocol for FGF9 antibody 26554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols