验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, BxPC-3 cells |
| Positive IF/ICC detected in | BxPC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
13254-1-AP targets FGFBP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | FGFBP2 fusion protein Ag4036 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibroblast growth factor binding protein 2 |
| 别名 | HBp17-related protein, Fibroblast growth factor-binding protein 2, FGFBP-2, FGF-BP2, FGF-binding protein 2 |
| 计算分子量 | 223 aa, 28 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC025720 |
| 基因名称 | FGFBP2 |
| Gene ID (NCBI) | 83888 |
| RRID | AB_2103095 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYJ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibroblast growth factor-binding protein 2 (FGFBP2), also known as 37 kDa killer-specific secretory protein (KSP37), is a member of the fibroblast growth factor binding protein family. FGFBP2 was identified as a Th1/Tc1 specific secretory protein is expressed preferentially in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells and might be involved in essential processes of CTL-mediated immunity. An increase expression of FGFBP2 may be associated with atopic asthma and mild extrinsic asthma. Recently, an observation revealed that FGFBP2 correlate strongly with histology, stage and outcome in ovarian, and it might be a potential prognostic biomarker. The observed MW of FGFBP2 (37 kDa) varies from the predicated MW of 28 kDa, which may be due to the specific space conformation caused by internal disulfide bonds.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FGFBP2 antibody 13254-1-AP | Download protocol |
| IF protocol for FGFBP2 antibody 13254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
