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FGFBP3 Polyclonal antibody

FGFBP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24725-1-AP

Print datasheet

Synonyms

C10orf13, FGF binding protein 3, FGF BP3, FGFBP 3, FGFBP3

验证数据展示

  • WB analysis using 24725-1-AP

    WB analysis using 24725-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24725-1-AP (FGFBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 24725-1-AP

    IF Staining of A431 using 24725-1-AP

    Immunofluorescent analysis of A431 cells using 24725-1-AP (FGFBP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24725-1-AP is FGFBP3 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FGFBP3 fusion protein Ag20573 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibroblast growth factor binding protein 3
Synonyms C10orf13, FGF binding protein 3, FGF BP3, FGFBP 3, FGFBP3
Calculated Molecular Weight 258 aa, 28 kDa
Observed Molecular Weight 60-65 kda
GenBank Accession NumberBC025966
Gene Symbol FGFBP3
Gene ID (NCBI) 143282
RRIDAB_2879691
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGFBP3 also named as C10orf13 or FGF binding protein 3 is 258 amino acid protein, which belongs to the fibroblast growth factor binding family. FGFBP3 is a secreted protein and may play a role in the regulation of gene transcription. The molecular weight of FGFBP3 is 29kDa, but our results detected the 60-65kDa protein by western blot. We infer that FGFBP3 may exist as dimer.

实验方案

Product Specific Protocols
WB protocol for FGFBP3 antibody 24725-1-APDownload protocol
IF protocol for FGFBP3 antibody 24725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols