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验证数据展示

  • IF Staining of ACHN using 60325-1-Ig (same clone as 60325-1-PBS)

    IF Staining of ACHN using 60325-1-Ig (same clone as 60325-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed ACHN cells using FGFR1 antibody (60325-1-Ig, Clone: 10F12E7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 60325-1-Ig (same clone as 60325-1-PBS)

    FC experiment of K-562 using 60325-1-Ig (same clone as 60325-1-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.2 ug FGFR1 Monoclonal antibody (60325-1-Ig, Clone:10F12E7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 60325-1-Ig (same clone as 60325-1-PBS)

    WB analysis of MCF-7 using 60325-1-Ig (same clone as 60325-1-PBS)

    MCF-7, HT-1080, HUVEC, U2OS, Y79, and K-562 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig (FGFR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60325-1-Ig (same clone as 60325-1-PBS)

    IHC staining of human breast cancer using 60325-1-Ig (same clone as 60325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60325-1-Ig (same clone as 60325-1-PBS)

    IHC staining of human breast cancer using 60325-1-Ig (same clone as 60325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50453-3

    Cytometric bead array standard curve of MP50453-3

    Cytometric bead array standard curve of MP50453-3, FGFR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68982-1-PBS. Detection antibody: 60325-1-PBS. Standard:Ag31801. Range: 1.563-100 ng/mL.

FGFR1 Monoclonal antibody, PBS Only (Detector)

FGFR1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 60325-1-PBS

产品说明书

CloneNo. 10F12E7

宿主/亚型

Mouse / IgG2c

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

EC:2.7.10.1, CEK, CD331, bFGF-R-1, BFGFR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60325-1-PBS targets FGFR1 as part of a matched antibody pair:

MP50453-3: 68982-1-PBS capture and 60325-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 FGFR1 fusion protein Ag3646 种属同源性预测
宿主/亚型 Mouse / IgG2c
抗体类别 Monoclonal
产品类型 Antibody
全称 fibroblast growth factor receptor 1
别名 EC:2.7.10.1, CEK, CD331, bFGF-R-1, BFGFR
计算分子量 820 aa, 91 kDa
观测分子量 91 kDa
GenBank蛋白编号BC018128
基因名称 FGFR1
Gene ID (NCBI) 2260
RRIDAB_2881435
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11362
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.