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FGFR2 Polyclonal antibody, PBS Only

FGFR2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 13042-1-PBS

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Synonyms

验证数据展示

  • IHC staining of human breast cancer using 13042-1-AP (same clone as 13042-1-PBS)

    IHC staining of human breast cancer using 13042-1-AP (same clone as 13042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13042-1-AP (same clone as 13042-1-PBS)

    IF Staining of HeLa using 13042-1-AP (same clone as 13042-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FGFR2 antibody (13042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

    FC experiment of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human FGFR2 (13042-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

产品信息

13042-1-PBS targets FGFR2 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen FGFR2 fusion protein Ag3701 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibroblast growth factor receptor 2
Synonyms
Calculated Molecular Weight 709 aa, 79 kDa
Observed Molecular Weight
GenBank Accession NumberBC039243
Gene Symbol FGFR2
Gene ID (NCBI) 2263
RRIDAB_10642943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21802
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. This polyclonal antibody raised against 358-704aa of human FGFR2 can cross-react with other members of the FGFR family.