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验证数据展示

  • WB analysis using 66954-1-Ig (same clone as 66954-1-PBS)

    WB analysis using 66954-1-Ig (same clone as 66954-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

    IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

    IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • WB analysis of L-929 using 66954-1-Ig (same clone as 66954-1-PBS)

    WB analysis of L-929 using 66954-1-Ig (same clone as 66954-1-PBS)

    L-929 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50578-1

    Cytometric bead array standard curve of MP50578-1

    Cytometric bead array standard curve of MP50578-1, FGFR3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66954-1-PBS. Detection antibody: 66954-2-PBS. Standard:Ag26290. Range: 1.563-200 ng/mL.

  • Featured Product
  • KD/KO验证

FGFR3 Monoclonal antibody, PBS Only (Capture)

FGFR3 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66954-1-PBS

产品说明书

CloneNo. 1F3G1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

JTK4, HSFGFR3EX, FGFR 3, CEK2, CD333

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FGFR3 Ready-To-Use IHC Kit. FGFR3 primary antibody included.

产品信息

66954-1-PBS targets FGFR3 as part of a matched antibody pair:

MP50578-1: 66954-1-PBS capture and 66954-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 FGFR3 fusion protein Ag26290 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fibroblast growth factor receptor 3
别名 JTK4, HSFGFR3EX, FGFR 3, CEK2, CD333
计算分子量 87 kDa
观测分子量 125-135 kDa
GenBank蛋白编号NM_000142
基因名称 FGFR3
Gene ID (NCBI) 2261
RRIDAB_2882277
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3, and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, the FGFR3 gene has also been associated with tumor progression.