验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, human ileum tissue, mouse thymus tissue, rat thymus tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11827-1-AP targets FGL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | FGL2 fusion protein Ag2395 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibrinogen-like 2 |
| 别名 | Fibroleukin, Fibrinogen-like protein 2, Fibrinogen like protein 2, fibrinogen like 2, FGL-2 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 70 kDa, 50-60 kDa |
| GenBank蛋白编号 | BC017813 |
| 基因名称 | FGL2 |
| Gene ID (NCBI) | 10875 |
| RRID | AB_2103947 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14314 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FGL2 antibody 11827-1-AP | Download protocol |
| IHC protocol for FGL2 antibody 11827-1-AP | Download protocol |
| IF protocol for FGL2 antibody 11827-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia. | ||
Int Immunopharmacol Identification of integrative molecular and clinical profiles of Fibrinogen-like protein 2 in gliomas using 1323 samples. | ||
Mol Immunol IL-33 protects murine viral fulminant hepatitis by targeting coagulation hallmark protein FGL2/fibroleukin expression. | ||
Oncoimmunology Integrative analysis identifies an immune-relevant epigenetic signature for prognostication of non-G-CIMP glioblastomas. | ||
Cell Metab Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain | ||
Phytomedicine Cuyun Recipe ameliorates pregnancy loss by regulating macrophage polarization and hypercoagulable state during the peri-implantation period in an ovarian hyperstimulation mouse model |
