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验证数据展示

  • WB analysis using 31641-1-AP

    WB analysis using 31641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31641-1-AP (FGR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 31641-1-AP

    IF Staining of Raji using 31641-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using FGR antibody (31641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FGR Polyclonal antibody

FGR Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31641-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Proto oncogene c Fgr, p58c fgr, p55c fgr, p55 Fgr, c src2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, Raji cells
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31641-1-AP targets FGR in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FGR fusion protein Ag34944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
别名 Proto oncogene c Fgr, p58c fgr, p55c fgr, p55 Fgr, c src2
计算分子量59 kDa
观测分子量59 kDa
GenBank蛋白编号NM_005248
基因名称 FGR
Gene ID (NCBI) 2268
RRIDAB_3670061
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP09769
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGR (Tyrosine-protein kinase Fgr), a member of the Src family of tyrosine kinases, is highly expressed in many cancers and can regulate mitochondrial oxidative phosphorylation (PMID: 37475042).

实验方案

Product Specific Protocols
WB protocol for FGR antibody 31641-1-APDownload protocol
IF protocol for FGR antibody 31641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols