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FHIT Polyclonal antibody

FHIT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13362-1-AP

Print datasheet

Synonyms

AP3A hydrolase, AP3Aase, Bis(5 adenosyl) triphosphatase, Dinucleosidetriphosphatase, FHIT, FRA3B, fragile histidine triad gene

验证数据展示

  • WB analysis using 13362-1-AP

    WB analysis using 13362-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13362-1-AP (FHIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HL-60 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13362-1-AP targets FHIT in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen FHIT fusion protein Ag4181 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fragile histidine triad gene
Synonyms AP3A hydrolase, AP3Aase, Bis(5 adenosyl) triphosphatase, Dinucleosidetriphosphatase, FHIT, FRA3B, fragile histidine triad gene
Calculated Molecular Weight 147 aa, 17 kDa
Observed Molecular Weight15-17 kDa
GenBank Accession NumberBC032336
Gene Symbol FHIT
Gene ID (NCBI) 2272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49789
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.

实验方案

Product Specific Protocols
WB protocol for FHIT antibody 13362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols