验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U2OS cells, hTERT-RPE1 cells, NIH/3T3 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human cervical cancer tissue, human skin cancer tissue, mouse kidney tissue, mouse ovary tissue, rat heart tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy FHL2 Ready-To-Use IHC Kit. FHL2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21619-1-AP targets FHL2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | FHL2 fusion protein Ag1378 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | four and a half LIM domains 2 |
| 别名 | Skeletal muscle LIM protein 3, LIM domain protein DRAL, FHL-2, FHL 2, DRAL |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC014397 |
| 基因名称 | FHL2 |
| Gene ID (NCBI) | 2274 |
| RRID | AB_10860263 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14192 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FHL2 antibody 21619-1-AP | Download protocol |
| IHC protocol for FHL2 antibody 21619-1-AP | Download protocol |
| IF protocol for FHL2 antibody 21619-1-AP | Download protocol |
| IP protocol for FHL2 antibody 21619-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. | ||
EBioMedicine Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome. | ||
Cell Death Dis FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells
| ||
iScience Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice | ||
Int J Mol Sci FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3
| ||
J Virol Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein. |