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验证数据展示

  • WB analysis of BxPC-3 using 15968-1-AP

    WB analysis of BxPC-3 using 15968-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15968-1-AP

    IF Staining of HepG2 using 15968-1-AP

    Immunofluorescent analysis of HepG2 cells using 15968-1-AP (FIBP antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human kidney using 15968-1-AP

    IHC staining of human kidney using 15968-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15968-1-AP (FIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FIBP Polyclonal antibody

FIBP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15968-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

FGFIBP, FIBP, FIBP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, mouse pancreas tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15968-1-AP targets FIBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FIBP fusion protein Ag8743 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibroblast growth factor (acidic) intracellular binding protein
别名 FGFIBP, FIBP, FIBP 1
计算分子量 357 aa, 41 kDa
观测分子量 42 kDa
GenBank蛋白编号BC014388
基因名称 FIBP
Gene ID (NCBI) 9158
RRIDAB_2878203
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43427
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FIBP, also named as Acidic fibroblast growth factor intracellular binding protein, is a 364 amino acid protein, which is highly expressed in heart, skeletal muscle and pancreas. FIBP may be involved in mitogenic function of FGF1.

实验方案

Product Specific Protocols
WB protocol for FIBP antibody 15968-1-APDownload protocol
IHC protocol for FIBP antibody 15968-1-APDownload protocol
IF protocol for FIBP antibody 15968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols