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验证数据展示

  • WB analysis of A549 using 11974-1-AP

    WB analysis of A549 using 11974-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11974-1-AP (FICD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 11974-1-AP

    IHC staining of human kidney using 11974-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human small intestine using 11974-1-AP

    IHC staining of human small intestine using 11974-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 40x lens).

FICD Polyclonal antibody

FICD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11974-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AMPylator FICD, FIC domain containing, FIC domain containing protein, FICD, HIP 13, HIP13, Huntingtin yeast partner E, HYPE, UNQ3041

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Jurkat cells
Positive IHC detected inhuman small intestine tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11974-1-AP targets FICD in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FICD fusion protein Ag2597 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FIC domain containing
别名 AMPylator FICD, FIC domain containing, FIC domain containing protein, FICD, HIP 13, HIP13, Huntingtin yeast partner E, HYPE, UNQ3041
计算分子量 458 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC001342
基因名称 FICD
Gene ID (NCBI) 11153
RRIDAB_2877811
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BVA6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenosine monophosphate-protein transferase FICD, also named as HIP13 or HYPE, is a 458 amino acid protein, which contains one fido domain and two TPR repeats. FICD localizes in the membrane and belongs to the fic family. FICD as an adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. The fido domain mediates the adenylyltransferase activity

实验方案

Product Specific Protocols
WB protocol for FICD antibody 11974-1-APDownload protocol
IHC protocol for FICD antibody 11974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols