• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FICD Polyclonal antibody

FICD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11974-1-AP

Print datasheet

Synonyms

AMPylator FICD, FIC domain containing, FIC domain containing protein, FICD, HIP 13, HIP13, Huntingtin yeast partner E, HYPE, UNQ3041

验证数据展示

  • WB analysis of A549 using 11974-1-AP

    WB analysis of A549 using 11974-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11974-1-AP (FICD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 11974-1-AP

    IHC staining of human kidney using 11974-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human small intestine using 11974-1-AP

    IHC staining of human small intestine using 11974-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA549 cells, Jurkat cells
Positive IHC detected inhuman small intestine tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11974-1-AP targets FICD in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FICD fusion protein Ag2597 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FIC domain containing
Synonyms AMPylator FICD, FIC domain containing, FIC domain containing protein, FICD, HIP 13, HIP13, Huntingtin yeast partner E, HYPE, UNQ3041
Calculated Molecular Weight 458 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC001342
Gene Symbol FICD
Gene ID (NCBI) 11153
RRIDAB_2877811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVA6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenosine monophosphate-protein transferase FICD, also named as HIP13 or HYPE, is a 458 amino acid protein, which contains one fido domain and two TPR repeats. FICD localizes in the membrane and belongs to the fic family. FICD as an adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. The fido domain mediates the adenylyltransferase activity

实验方案

Product Specific Protocols
WB protocol for FICD antibody 11974-1-APDownload protocol
IHC protocol for FICD antibody 11974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols