验证数据展示
经过测试的应用
| Positive WB detected in | FITC-TDP43, FITC-Ag1231, BSA |
| Positive ELISA detected in | FITC conjugated antibody |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68132-1-Ig targets FITC in WB, ELISA applications and shows reactivity with chemical compound, fitc samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | chemical compound, fitc |
| Cited Reactivity | mouse |
| Immunogen | Compound 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | FITC |
| Synonyms | Fluorescein isothiocyanate |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2923659 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FITC antibody 68132-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |