验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, human brain tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 3 publications below |
产品信息
15884-1-AP targets FKBP14 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | FKBP14 fusion protein Ag8668 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FK506 binding protein 14, 22 kDa |
| 别名 | FKBP-22, FKBP22, FKBP-14, FK506-binding protein 14, EC:5.2.1.8 |
| 计算分子量 | 211 aa, 24 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC005206 |
| 基因名称 | FKBP14 |
| Gene ID (NCBI) | 55033 |
| RRID | AB_2102702 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NWM8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FKBP14, also named as FKBP22, belongs to the family of FK506-binding peptidyl-prolyl cis-trans isomerases (PPIases). PPIases accelerate the folding of proteins during protein synthesis. FKBP14 is localized in the endoplasmic reticulum (ER) and that deficiency of FKBP14 leads to enlarged ER cisterns in dermal fibroblasts in vivo. FKBP14 is about 22kd.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FKBP14 antibody 15884-1-AP | Download protocol |
| IHC protocol for FKBP14 antibody 15884-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Mutations in FKBP14 cause a variant of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss. | ||
Sci Rep The novel missense mutation Met48Lys in FKBP22 changes its structure and functions. | ||
Hum Mutat Kyphoscoliotic Ehlers-Danlos syndrome caused by pathogenic variants in FKBP14: Further insights into the phenotypic spectrum and pathogenic mechanisms | ||
Int J Mol Sci Local Net Charge State of Collagen Triple Helix Is a Determinant of FKBP22 Binding to Collagen III |
