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验证数据展示

  • WB analysis of HeLa using 66040-1-Ig

    WB analysis of HeLa using 66040-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 66040-1-Ig

    IHC staining of human cervical cancer using 66040-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

    Ready-to-use IHC kits available with FKBP4/FKBP52 antibody included
  • IHC staining of human cervical cancer using 66040-1-Ig

    IHC staining of human cervical cancer using 66040-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

    Ready-to-use IHC kits available with FKBP4/FKBP52 antibody included
  • IF Staining of HeLa using 66040-1-Ig

    IF Staining of HeLa using 66040-1-Ig

    Immunofluorescent analysis of HeLa cells using 66040-1-Ig (FKBP52 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

FKBP52 Monoclonal antibody

FKBP52 Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 66040-1-Ig
CloneNo. 3F5F9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Synonyms

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, K-562 cells
Positive IHC detected inhuman cervical cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FKBP4/FKBP52 Ready-To-Use IHC Kit. FKBP4/FKBP52 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66040-1-Ig targets FKBP52 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FKBP52 fusion protein Ag18704 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name FK506 binding protein 4, 59kDa
Synonyms 51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007924
Gene Symbol FKBP52
Gene ID (NCBI) 2288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.

实验方案

Product Specific Protocols
WB protocol for FKBP52 antibody 66040-1-IgDownload protocol
IHC protocol for FKBP52 antibody 66040-1-IgDownload protocol
IF protocol for FKBP52 antibody 66040-1-IgDownload protocol
FC protocol for FKBP52 antibody 66040-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols