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  • Featured Product
  • KD/KO Validated

FKBP8 Polyclonal antibody

FKBP8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11173-1-AP

Print datasheet

Synonyms

FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8

验证数据展示

  • WB analysis of HeLa using 11173-1-AP

    WB analysis of HeLa using 11173-1-AP

    WB result of FKBP8 antibody (11173-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP8 transfected HeLa cells.

  • WB analysis using 11173-1-AP

    WB analysis using 11173-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 11173-1-AP

    IP experiment of HeLa using 11173-1-AP

    IP result of anti-FKBP8 (IP:11173-1-AP, 4ug; Detection:11173-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IF Staining of SH-SY5Y using 11173-1-AP

    IF Staining of SH-SY5Y using 11173-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 11173-1-AP (FKBP8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse pancreas tissue, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11173-1-AP targets FKBP8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen FKBP8 fusion protein Ag1649 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FK506 binding protein 8, 38kDa
Synonyms FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC009966
Gene Symbol FKBP8
Gene ID (NCBI) 23770
RRIDAB_10597097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for FKBP8 antibody 11173-1-APDownload protocol
IF protocol for FKBP8 antibody 11173-1-APDownload protocol
IP protocol for FKBP8 antibody 11173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Elife

ZMYND10 functions in a chaperone relay during axonemal dynein assembly.

Authors - Girish R Mali
View Article
human

Front Bioeng Biotechnol

Efficient and reversible Cas13d-mediated knockdown with an all-in-one lentivirus-vector

Authors - Suli Lv
  • KO Validated
View Article
ratIF

Int J Mol Sci

Analysis of Dysferlin Direct Interactions with Putative Repair Proteins Links Apoptotic Signaling to Ca2+ Elevation via PDCD6 and FKBP8

Authors - Dennis G Drescher
View Article
humanWB

J Biol Chem

Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma

Authors - Suli Lv
  • KO Validated
View Article
humanWB

Mol Hum Reprod

Comprehensive analysis of ovarian granulosa cell proteomics and phosphoproteomics in PCOS patients without insulin resistance

Authors - Xiao Yang
View Article