验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse pancreas tissue, Jurkat cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11173-1-AP targets FKBP8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | FKBP8 fusion protein Ag1649 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FK506 binding protein 8, 38kDa |
| 别名 | FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC009966 |
| 基因名称 | FKBP8 |
| Gene ID (NCBI) | 23770 |
| RRID | AB_10597097 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14318 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FKBP8 antibody 11173-1-AP | Download protocol |
| IF protocol for FKBP8 antibody 11173-1-AP | Download protocol |
| IP protocol for FKBP8 antibody 11173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Bioeng Biotechnol Efficient and reversible Cas13d-mediated knockdown with an all-in-one lentivirus-vector
| ||
Int J Mol Sci Analysis of Dysferlin Direct Interactions with Putative Repair Proteins Links Apoptotic Signaling to Ca2+ Elevation via PDCD6 and FKBP8 | ||
J Biol Chem Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma
| ||
Mol Hum Reprod Comprehensive analysis of ovarian granulosa cell proteomics and phosphoproteomics in PCOS patients without insulin resistance |
