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FKBPL Polyclonal antibody

FKBPL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 23369-1-AP

Print datasheet

Synonyms

WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1

验证数据展示

  • WB analysis of HeLa using 23369-1-AP

    WB analysis of HeLa using 23369-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23369-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 23369-1-AP

    IF Staining of HeLa using 23369-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FKBPL antibody (23369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human ovary tumor using 23369-1-AP

    IHC staining of human ovary tumor using 23369-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23369-1-AP (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 23369-1-AP

    IHC staining of human ovary tumor using 23369-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23369-1-AP (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 23369-1-AP

    FC experiment of HeLa using 23369-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Polyclonal antibody (23369-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23369-1-AP targets FKBPL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen FKBPL fusion protein Ag19702 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FK506 binding protein like
Synonyms WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1
Calculated Molecular Weight 349 aa, 38 kDa
Observed Molecular Weight 42-48 kDa
GenBank Accession NumberBC004168
Gene Symbol FKBPL
Gene ID (NCBI) 63943
RRIDAB_2879267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.

实验方案

Product Specific Protocols
WB protocol for FKBPL antibody 23369-1-APDownload protocol
IHC protocol for FKBPL antibody 23369-1-APDownload protocol
IF protocol for FKBPL antibody 23369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols