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Fukutin Polyclonal antibody

Fukutin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18276-1-AP

Print datasheet

Synonyms

CMD1X, FCMD, FKTN, Fukutin, LGMD2M

验证数据展示

  • WB analysis of human brain using 18276-1-AP

    WB analysis of human brain using 18276-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18276-1-AP (Fukutin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 18276-1-AP

    IHC staining of human pancreas using 18276-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18276-1-AP (Fukutin antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas using 18276-1-AP

    IHC staining of human pancreas using 18276-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18276-1-AP (Fukutin antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse brain using 18276-1-AP

    IP experiment of mouse brain using 18276-1-AP

    IP result of anti-Fukutin (IP:18276-1-AP, 4ug; Detection:18276-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18276-1-AP targets Fukutin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Fukutin fusion protein Ag13067 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fukutin
Synonyms CMD1X, FCMD, FKTN, Fukutin, LGMD2M
Calculated Molecular Weight 461 aa, 54 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC101808
Gene Symbol Fukutin
Gene ID (NCBI) 2218
RRIDAB_2103278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75072
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Fukutin antibody 18276-1-APDownload protocol
IHC protocol for Fukutin antibody 18276-1-APDownload protocol
IP protocol for Fukutin antibody 18276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Direct mapping of additional modifications on phosphorylated O-glycans of α-dystroglycan by mass spectrometry analysis in conjunction with knocking out of causative genes for dystroglycanopathy.

Authors - Hirokazu Yagi
View Article