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验证数据展示

  • WB analysis of human brain using 18276-1-AP

    WB analysis of human brain using 18276-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18276-1-AP (Fukutin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 18276-1-AP

    IHC staining of human pancreas using 18276-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18276-1-AP (Fukutin antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas using 18276-1-AP

    IHC staining of human pancreas using 18276-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18276-1-AP (Fukutin antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse brain using 18276-1-AP

    IP experiment of mouse brain using 18276-1-AP

    IP result of anti-Fukutin (IP:18276-1-AP, 4ug; Detection:18276-1-AP 1:500) with mouse brain tissue lysate 4000ug.

Fukutin Polyclonal antibody

Fukutin Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 18276-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

CMD1X, FCMD, FKTN, Fukutin, LGMD2M

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18276-1-AP targets Fukutin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Fukutin fusion protein Ag13067 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fukutin
别名 CMD1X, FCMD, FKTN, Fukutin, LGMD2M
计算分子量 461 aa, 54 kDa
观测分子量 60 kDa
GenBank蛋白编号BC101808
基因名称 Fukutin
Gene ID (NCBI) 2218
RRIDAB_2103278
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75072
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Fukutin antibody 18276-1-APDownload protocol
IHC protocol for Fukutin antibody 18276-1-APDownload protocol
IP protocol for Fukutin antibody 18276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Direct mapping of additional modifications on phosphorylated O-glycans of α-dystroglycan by mass spectrometry analysis in conjunction with knocking out of causative genes for dystroglycanopathy.

Authors - Hirokazu Yagi
View Article