验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HEK-293 cells, HepG2 cells, rat liver tissue, L02 cells, mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy FLAD1 Ready-To-Use IHC Kit. FLAD1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
14118-1-AP targets FLAD1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | FLAD1 fusion protein Ag5267 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) |
| 别名 | FADS, FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase |
| 计算分子量 | 446 aa, 49 kDa |
| 观测分子量 | 50 kDa, 65-70 kDa |
| GenBank蛋白编号 | BC011378 |
| 基因名称 | FLAD1 |
| Gene ID (NCBI) | 80308 |
| RRID | AB_2878017 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NFF5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FLAD1 antibody 14118-1-AP | Download protocol |
| IF protocol for FLAD1 antibody 14118-1-AP | Download protocol |
| IP protocol for FLAD1 antibody 14118-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
