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AAGAB Polyclonal antibody

AAGAB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27319-1-AP

Print datasheet

Synonyms

AAGAB, FLJ11506

验证数据展示

  • IHC staining of mouse thymus using 27319-1-AP

    IHC staining of mouse thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AAGAB antibody included
  • IHC staining of mouse thymus using 27319-1-AP

    IHC staining of mouse thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AAGAB antibody included
  • WB analysis of K-562 using 27319-1-AP

    WB analysis of K-562 using 27319-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27319-1-AP (AAGAB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, mouse thymus tissue
Positive IHC detected inmouse thymus tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AAGAB Ready-To-Use IHC Kit. AAGAB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27319-1-AP targets AAGAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AAGAB fusion protein Ag26340 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alpha- and gamma-adaptin-binding protein p34
Synonyms AAGAB, FLJ11506
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC058886
Gene Symbol AAGAB
Gene ID (NCBI) 79719
RRIDAB_2880841
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PD74
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Recent study indicate that mutations in AAGAB can cause punctate palmoplantar keratoderma type I (PPKP1; OMIM#148600), also called keratosis punctata palmoplantaris Buschke-Fischer-Brauer. The predicted MW of this protein is 35 kDa. Catalog#27319-1-AP recognises 37-40 kDa band may due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for AAGAB antibody 27319-1-APDownload protocol
IHC protocol for AAGAB antibody 27319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols