Filamin B Polyclonal antibody
Filamin B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U2OS cells, NIH/3T3 cells |
| Positive IHC detected in | human liver cancer tissue, human normal colon, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20685-1-AP targets Filamin B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | filamin B, beta (actin binding protein 278) |
| Synonyms | FLNB, FilaminB, Filamin-3, Beta-filamin, Actin-binding-like protein |
| Calculated Molecular Weight | 278 kDa |
| Observed Molecular Weight | 250-278 kDa |
| GenBank Accession Number | NM_001457 |
| Gene Symbol | FLNB |
| Gene ID (NCBI) | 2317 |
| RRID | AB_10863658 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75369 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. The antibody is specific to FLNB. It can recognize isoform1,2,3,6,7 of FLNB. It has no cross reaction to FLNA and FLNC.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Filamin B antibody 20685-1-AP | Download protocol |
| IHC protocol for Filamin B antibody 20685-1-AP | Download protocol |
| IF protocol for Filamin B antibody 20685-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90. | ||
Heliyon Filamin B knockdown impairs differentiation and function in mouse pre-osteoblasts via aberrant transcription and alternative splicing
| ||
J Genet Genomics Rare loss-of-function variants in FLNB cause non-syndromic orofacial clefts | ||
Am J Cancer Res FLNB overexpression promotes tumor progression and associates with immune suppression, evasion and stemness in pancreatic cancer |
