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FMN1 Polyclonal antibody

FMN1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25982-1-AP

Print datasheet

Synonyms

FMN, FMN1, FMN1 Ens, formin 1, LD

验证数据展示

  • WB analysis of A549 using 25982-1-AP

    WB analysis of A549 using 25982-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25982-1-AP (FMN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 25982-1-AP

    IF Staining of A549 using 25982-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 25982-1-AP (FMN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells
Positive IF detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25982-1-AP is FMN1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen FMN1 fusion protein Ag23059 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name formin 1
Synonyms FMN, FMN1, FMN1 Ens, formin 1, LD
Calculated Molecular Weight 1196 aa, 132 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC103692
Gene Symbol FMN1
Gene ID (NCBI) 342184
RRIDAB_2880319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68DA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FMN1 antibody 25982-1-APDownload protocol
IF protocol for FMN1 antibody 25982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Molecules

MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway

Authors - Pan Xu
View Article