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FMNL1 Polyclonal antibody

FMNL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10395-1-AP

Print datasheet

Synonyms

Formin-like protein 1, FMNL, CLL-associated antigen KW-13

验证数据展示

  • WB analysis of Raji using 10395-1-AP

    WB analysis of Raji using 10395-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 10395-1-AP

    IHC staining of human small intestine using 10395-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10395-1-AP (FMNL1 antibody) at dilution of 1:200 (under 10x lens).

  • IF Staining of U2OS using 10395-1-AP

    IF Staining of U2OS using 10395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FMNL1 antibody (10395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of Saos-2 using 10395-1-AP

    IF Staining of Saos-2 using 10395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using FMNL1 antibody (10395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 10395-1-AP

    WB analysis using 10395-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, Saos-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:500
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10395-1-AP targets FMNL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen FMNL1 fusion protein Ag0594 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name formin-like 1
Synonyms Formin-like protein 1, FMNL, CLL-associated antigen KW-13
Calculated Molecular Weight 160 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC001710
Gene Symbol FMNL1
Gene ID (NCBI) 752
RRIDAB_10895969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95466
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. The MW of FMNL1 is about 150-160 kDa. (PMID: 16494944 )

实验方案

Product Specific Protocols
WB protocol for FMNL1 antibody 10395-1-APDownload protocol
IHC protocol for FMNL1 antibody 10395-1-APDownload protocol
IF protocol for FMNL1 antibody 10395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols