验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, Jurkat cells, HeLa cells |
| Positive IP detected in | Caki-1 |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27834-1-AP targets FMNL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 | FMNL1 fusion protein Ag27312 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | formin-like 1 |
| 别名 | C17orf1, C17orf1B, CLL associated antigen KW 13, FHOD4, FMNL, FMNL1, formin like 1, Formin like protein 1, KW 13, Leukocyte formin |
| 观测分子量 | 125-150 kDa |
| GenBank蛋白编号 | BC111564 |
| 基因名称 | FMNL1 |
| Gene ID (NCBI) | 752 |
| RRID | AB_2880987 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95466 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 27834-1-AP is a rabbit polyclonal antibody recognises the 125-150 kDa protein.(PMID:16494944)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FMNL1 antibody 27834-1-AP | Download protocol |
| IHC protocol for FMNL1 antibody 27834-1-AP | Download protocol |
| IF protocol for FMNL1 antibody 27834-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer
| ||
Gastric Cancer FHOD1 and FMNL1 formin proteins in intestinal gastric cancer: correlation with tumor-infiltrating T lymphocytes and molecular subtypes. | ||
Cell Rep Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction. | ||
Biomed Res Int Systematic Characterization of Expression Patterns and Immunocorrelations of Formin-Like Genes in Breast Cancer | ||
Clin Immunol Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy | ||
J Cancer Formin protein FMNL1 is a biomarker for tumor-infiltrating immune cells and associated with well immunotherapeutic response |
