验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, NK-92 cells, HL-60 cells, K-562 cells, pig thymus tissue |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67587-1-Ig targets FMNL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, pig |
| 免疫原 | FMNL1 fusion protein Ag27322 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | formin-like 1 |
| 别名 | Formin-like protein 1, FMNL, CLL-associated antigen KW-13, 1B12A4 |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC111564 |
| 基因名称 | FMNL1 |
| Gene ID (NCBI) | 752 |
| RRID | AB_2882796 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95466 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 67587-1-Ig antibody detects the protein around 140-150 kDa which is similar to the results reported in publications.(PMID: 21868368, 22986496 , 23182705)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FMNL1 antibody 67587-1-Ig | Download protocol |
| IF protocol for FMNL1 antibody 67587-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |