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验证数据展示

  • IHC staining of human colon cancer using 29756-1-AP

    IHC staining of human colon cancer using 29756-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FMNL3 antibody included
  • IHC staining of human colon cancer using 29756-1-AP

    IHC staining of human colon cancer using 29756-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FMNL3 antibody included
  • WB analysis using 29756-1-AP

    WB analysis using 29756-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29756-1-AP (FMNL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 29756-1-AP

    IF Staining of HUVEC using 29756-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FMNL3 antibody (29756-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

FMNL3 Polyclonal antibody

FMNL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29756-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

DKFZp762B245, FHOD3, FLJ45265, FMNL3, formin like 3, Formin like protein 3, KIAA2014, WBP 3, WBP3, WW domain binding protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy FMNL3 Ready-To-Use IHC Kit. FMNL3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29756-1-AP targets FMNL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 FMNL3 fusion protein Ag30543 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 formin-like 3
别名 DKFZp762B245, FHOD3, FLJ45265, FMNL3, formin like 3, Formin like protein 3, KIAA2014, WBP 3, WBP3, WW domain binding protein 3
计算分子量 117 kDa
观测分子量117 kDa
GenBank蛋白编号NM_175736
基因名称 FMNL3
Gene ID (NCBI) 91010
RRIDAB_3086155
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVF7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FMNL3 plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the control of cell shape and migration. In quiescent endothelial cells, FMNL3 triggers rearrangement of the actin cytoskeleton, but does not alter microtubule alignement.

实验方案

Product Specific Protocols
WB protocol for FMNL3 antibody 29756-1-APDownload protocol
IHC protocol for FMNL3 antibody 29756-1-APDownload protocol
IF protocol for FMNL3 antibody 29756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols