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验证数据展示

  • IHC staining of human liver using 30977-1-AP

    IHC staining of human liver using 30977-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30977-1-AP (FMO1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 30977-1-AP

    IHC staining of human liver using 30977-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30977-1-AP (FMO1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FMO1 Polyclonal antibody

FMO1 Polyclonal Antibody for IHC, ELISA
Cat No. 30977-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

Trimethylamine monooxygenase, Flavin-containing monooxygenase 1, Fetal hepatic flavin-containing monooxygenase 1, EC:1.14.13.148, Dimethylaniline monooxygenase [N-oxide-forming] 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30977-1-AP targets FMO1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FMO1 fusion protein Ag34267 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 flavin containing monooxygenase 1
别名 Trimethylamine monooxygenase, Flavin-containing monooxygenase 1, Fetal hepatic flavin-containing monooxygenase 1, EC:1.14.13.148, Dimethylaniline monooxygenase [N-oxide-forming] 1
计算分子量60 kDa
GenBank蛋白编号NM_001282692
基因名称 FMO1
Gene ID (NCBI) 2326
RRIDAB_3669802
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ01740
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Flavin-containing monooxygenase 1 (FMO1) belongs to the FMO family. FMO1 is an enzyme that catalyzes the oxygenation of a series of sulfur-containing and nitrogen-containing substrates, and thus is involved in a series of drugs and xenobiotics metabolism. FMO1 can regulate PPAR-α and mediate metabolic processes. FMO1 is expressed at relatively high levels in human fetal liver and also represents the major FMO enzyme in the adult human and rabbit intestine and kidney (PMID: 11809920, 30757917, 37553313). Human FMO1 has 2 isoforms with the molecular weights of 53 kDa and 60 kDa.

实验方案

Product Specific Protocols
IHC protocol for FMO1 antibody 30977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols