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验证数据展示

  • WB analysis using 15725-1-AP

    WB analysis using 15725-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15725-1-AP (FMO2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 15725-1-AP

    IHC staining of human lung cancer using 15725-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 15725-1-AP

    IHC staining of human lung cancer using 15725-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FMO2 Polyclonal antibody

FMO2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15725-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Dimethylaniline oxidase 2, FMO 1B1, FMO 2, FMO1B1, FMO2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15725-1-AP targets FMO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FMO2 fusion protein Ag8464 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 flavin containing monooxygenase 2
别名 Dimethylaniline oxidase 2, FMO 1B1, FMO 2, FMO1B1, FMO2
计算分子量471aa,54 kDa; 534aa,61 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC005894
基因名称 FMO2
Gene ID (NCBI) 2327
RRIDAB_2278518
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99518
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FMO2, belongs to FMO (flavin containing monooxygenase) family, which catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. FMO family has 1-5 members, with homologous sequences. This antibody is specially against FMO2. It does not bind other FMOs.

实验方案

Product Specific Protocols
WB protocol for FMO2 antibody 15725-1-APDownload protocol
IHC protocol for FMO2 antibody 15725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols