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FMO2 Polyclonal antibody

FMO2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15725-1-AP

Print datasheet

Synonyms

Dimethylaniline oxidase 2, FMO 1B1, FMO 2, FMO1B1, FMO2

验证数据展示

  • WB analysis using 15725-1-AP

    WB analysis using 15725-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15725-1-AP (FMO2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 15725-1-AP

    IHC staining of human lung cancer using 15725-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 15725-1-AP

    IHC staining of human lung cancer using 15725-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15725-1-AP targets FMO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FMO2 fusion protein Ag8464 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name flavin containing monooxygenase 2
Synonyms Dimethylaniline oxidase 2, FMO 1B1, FMO 2, FMO1B1, FMO2
Calculated Molecular Weight471aa,54 kDa; 534aa,61 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC005894
Gene Symbol FMO2
Gene ID (NCBI) 2327
RRIDAB_2278518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99518
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FMO2, belongs to FMO (flavin containing monooxygenase) family, which catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. FMO family has 1-5 members, with homologous sequences. This antibody is specially against FMO2. It does not bind other FMOs.

实验方案

Product Specific Protocols
WB protocol for FMO2 antibody 15725-1-APDownload protocol
IHC protocol for FMO2 antibody 15725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols