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  • Featured Product
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FMR1 Polyclonal antibody

FMR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 13755-1-AP

Print datasheet

Synonyms

POF, FRAXA, Fragile X messenger ribonucleoprotein 1, Fragile X messenger ribonucleoprotein, fragile X mental retardation 1

验证数据展示

  • WB analysis of Jurkat cells using 13755-1-AP

    WB analysis of Jurkat cells using 13755-1-AP

    WB result of FMR1 antibody (13755-1-AP, 1:1500) with si-Control and si-FMR1 transfected Jurkat cells. FMR1 gene has many isoforms with MW 59-72 kDa.

  • WB analysis of K-562 using 13755-1-AP

    WB analysis of K-562 using 13755-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 13755-1-AP

    IHC staining of human colon using 13755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FMR1 antibody included
  • IHC staining of human colon using 13755-1-AP

    IHC staining of human colon using 13755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FMR1 antibody included
  • FC experiment of Jurkat using 13755-1-AP

    FC experiment of Jurkat using 13755-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (13755-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human colon cancer using 13755-1-AP

    IHC staining of human colon cancer using 13755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FMR1 antibody included
  • IHC staining of human colon cancer using 13755-1-AP

    IHC staining of human colon cancer using 13755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FMR1 antibody included
  • IP experiment of HeLa using 13755-1-AP

    IP experiment of HeLa using 13755-1-AP

    IP result of anti-FMR1 (IP:13755-1-AP, 4ug; Detection:13755-1-AP 1:1000) with HeLa cells lysate 2800ug.

  • IF Staining of HepG2 using 13755-1-AP

    IF Staining of HepG2 using 13755-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13755-1-AP (FMRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, mouse brain tissue, mouse thymus tissue, Jurkat cells, HeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human liver tissue, human colon cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy FMR1 Ready-To-Use IHC Kit. FMR1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13755-1-AP targets FMR1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen FMR1 fusion protein Ag4697 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fragile X mental retardation 1
Synonyms POF, FRAXA, Fragile X messenger ribonucleoprotein 1, Fragile X messenger ribonucleoprotein, fragile X mental retardation 1
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 59-72 kDa
GenBank Accession NumberBC038998
Gene Symbol FMR1
Gene ID (NCBI) 2332
RRIDAB_2262872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

实验方案

Product Specific Protocols
WB protocol for FMR1 antibody 13755-1-APDownload protocol
IHC protocol for FMR1 antibody 13755-1-APDownload protocol
IF protocol for FMR1 antibody 13755-1-APDownload protocol
IP protocol for FMR1 antibody 13755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders
View Article
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
View Article
humanWB

Cell Rep

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

Authors - Xiaoli Wang
View Article
humanWB

Int Immunopharmacol

MNSFβ promotes LPS-induced TNFα expression by increasing the localization of RC3H1 to stress granules, and the interfering peptide HEPN2 reduces TNFα production by disrupting the MNSFβ-RC3H1 interaction in macrophages

Authors - Han-Yu Jiang
View Article
humanWB

Cancer Cell Int

MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome.

Authors - Yu Men
View Article
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
View Article