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FMR1NB Polyclonal antibody

FMR1NB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11069-2-AP

Print datasheet

Synonyms

Cancer/testis antigen 37, CT37, FMR1NB, NY SAR 35, Sarcoma antigen NY SAR 35

验证数据展示

  • WB analysis of MCF-7 using 11069-2-AP

    WB analysis of MCF-7 using 11069-2-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11069-2-AP

    IHC staining of mouse testis using 11069-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11069-2-AP (FMR1NB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF7 cells, A549 cells, MCF-7 cells, SKOV-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11069-2-AP targets FMR1NB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FMR1NB fusion protein Ag1531 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fragile X mental retardation 1 neighbor
Synonyms Cancer/testis antigen 37, CT37, FMR1NB, NY SAR 35, Sarcoma antigen NY SAR 35
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC034320
Gene Symbol FMR1NB
Gene ID (NCBI) 158521
RRIDAB_2105567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0W7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FMR1NB antibody 11069-2-APDownload protocol
IHC protocol for FMR1NB antibody 11069-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols