验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse kidney tissue, rat kidney tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16312-1-AP targets FN3KRP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | FN3KRP fusion protein Ag9347 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | fructosamine 3 kinase related protein |
别名 | Ketosamine-3-kinase, Fructosamine-3-kinase-related protein, FN3K-RP, FN3K-related protein, EC:2.7.1.172 |
计算分子量 | 309 aa, 34 kDa |
观测分子量 | 30-34 kDa |
GenBank蛋白编号 | BC014408 |
基因名称 | FN3KRP |
Gene ID (NCBI) | 79672 |
RRID | AB_2105986 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9HA64 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fn3KRP (Fn3K-related protein) are believed to be the first examples of deglycating enzymes acting on the Amadori product to reverse the early stages of the Maillard reaction in vivo in higher organisms (PMID: 17337043). Fn3KRP possesses an ATP-binding domain that is structurally related to that of both the aminoglycoside kinases and eukaryotic protein kinases. Fn3KRP possesses an intrinsic activity towards ketosamine substrates (PMID: 18637789). Catalog#16312-1-AP is a rabbit polyclonal antibody raised against the full-length of human FN3KRP. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.
实验方案
Product Specific Protocols | |
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WB protocol for FN3KRP antibody 16312-1-AP | Download protocol |
IF protocol for FN3KRP antibody 16312-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |