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验证数据展示

  • WB analysis using 16312-1-AP

    WB analysis using 16312-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16312-1-AP

    WB analysis of HEK-293 using 16312-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16312-1-AP

    IF Staining of HeLa using 16312-1-AP

    Immunofluorescent analysis of HeLa cells using 16312-1-AP (FN3KRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FN3KRP Polyclonal antibody

FN3KRP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16312-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Ketosamine-3-kinase, Fructosamine-3-kinase-related protein, FN3K-RP, FN3K-related protein, EC:2.7.1.172

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16312-1-AP targets FN3KRP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FN3KRP fusion protein Ag9347 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fructosamine 3 kinase related protein
Synonyms Ketosamine-3-kinase, Fructosamine-3-kinase-related protein, FN3K-RP, FN3K-related protein, EC:2.7.1.172
Calculated Molecular Weight 309 aa, 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC014408
Gene Symbol FN3KRP
Gene ID (NCBI) 79672
RRIDAB_2105986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HA64
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fn3KRP (Fn3K-related protein) are believed to be the first examples of deglycating enzymes acting on the Amadori product to reverse the early stages of the Maillard reaction in vivo in higher organisms (PMID: 17337043). Fn3KRP possesses an ATP-binding domain that is structurally related to that of both the aminoglycoside kinases and eukaryotic protein kinases. Fn3KRP possesses an intrinsic activity towards ketosamine substrates (PMID: 18637789). Catalog#16312-1-AP is a rabbit polyclonal antibody raised against the full-length of human FN3KRP. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.

实验方案

Product Specific Protocols
WB protocol for FN3KRP antibody 16312-1-APDownload protocol
IF protocol for FN3KRP antibody 16312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols