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验证数据展示

  • WB analysis of HEK-293 using 25240-1-AP

    WB analysis of HEK-293 using 25240-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25240-1-AP (FNDC3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 25240-1-AP

    IF Staining of U-251 using 25240-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using FNDC3A antibody (25240-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

FNDC3A Polyclonal antibody

FNDC3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25240-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

FNDC3, FNDC3A, HUGO, KIAA0970

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse placenta tissue
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25240-1-AP targets FNDC3A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FNDC3A fusion protein Ag18736 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibronectin type III domain containing 3A
别名 FNDC3, FNDC3A, HUGO, KIAA0970
计算分子量 1198 aa, 132 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC132812
基因名称 FNDC3A
Gene ID (NCBI) 22862
RRIDAB_2879982
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2H6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FNDC3A antibody 25240-1-APDownload protocol
IF protocol for FNDC3A antibody 25240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols