验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IF/ICC detected in | C2C12 cells |
Planning an IHC experiment? We recommend our IHCeasy FNIP1 Ready-To-Use IHC Kit. FNIP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
28380-1-AP targets FNIP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | FNIP1 fusion protein Ag28976 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | folliculin interacting protein 1 |
| 别名 | DKFZp686E18167, DKFZp781P0215, FNIP1, KIAA1961 |
| 计算分子量 | 508 aa, 57 kDa |
| 观测分子量 | 130-150 kDa |
| GenBank蛋白编号 | BC001956 |
| 基因名称 | FNIP1 |
| Gene ID (NCBI) | 96459 |
| RRID | AB_2881128 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TF40 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Folliculin-interacting protein 1 (FNIP1) forms a complex with the protein folliculin (FLCN), together acting as guanosine triphosphate (GTP)-activating protein (GAP) for RagC. The FNIP1-FLCN complex has emerged as an amino acid sensor to the mechanistic target of rapamycin complex 1 (mTORC1), involved in how amino acids control TFEB activation. AMPK phosphorylation of FNIP1 induces lysosomal and mitochondrial biogenesis (PMID: 37079666)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FNIP1 antibody 28380-1-AP | Download protocol |
| IF protocol for FNIP1 antibody 28380-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |