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验证数据展示

  • IP experiment of HEK-293 using 31283-1-AP

    IP experiment of HEK-293 using 31283-1-AP

    IP result of anti-FNIP2 (IP:31283-1-AP, 4ug; Detection:31283-1-AP 1:500) with HEK-293 cells lysate 1470 ug.

  • IHC staining of mouse kidney using 31283-1-AP

    IHC staining of mouse kidney using 31283-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31283-1-AP (FNIP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 31283-1-AP

    IHC staining of mouse kidney using 31283-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31283-1-AP (FNIP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FNIP2 Polyclonal antibody

FNIP2 Polyclonal Antibody for IP, IHC, ELISA
Cat No. 31283-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

IP, IHC, ELISA

别名

FNIP2, FNIPL, KIAA1450, FNIP1 like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31283-1-AP targets FNIP2 in IP, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 FNIP2 fusion protein Ag35014 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 folliculin interacting protein 2
别名 FNIP2, FNIPL, KIAA1450, FNIP1 like protein
观测分子量125 kDa
GenBank蛋白编号BC166693
基因名称 FNIP2
Gene ID (NCBI) 57600
RRIDAB_3669929
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Folliculin (FLCN)-interacting proteins 1 and 2 (FNIP1, FNIP2) are homologous binding partners of FLCN, a tumor suppressor for kidney cancer. FNIP2 was found to bind to the C terminus of FLCN and to AMPK, like FNIP1. FNIP2 competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PMID: 18403135).

实验方案

Product Specific Protocols
IHC protocol for FNIP2 antibody 31283-1-APDownload protocol
IP protocol for FNIP2 antibody 31283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols