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验证数据展示

  • WB analysis of A549 using 84371-2-RR

    WB analysis of A549 using 84371-2-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 84371-2-RR (FNIP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84371-2-RR

    Affinity and Kinetic Characterization of 84371-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84371-2-RR against Human FNIP2 were performed. The affinity constant is 4.85 nM.

FNIP2 Recombinant antibody

FNIP2 Recombinant Antibody for WB, ELISA
Cat No. 84371-2-RR

产品说明书

CloneNo. 241638F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

KIAA1450, FNIPL, FNIP1 like protein, 241638F2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MDA-MB-231 cells, MDA-MB-453 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84371-2-RR targets FNIP2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 FNIP2 fusion protein Ag35014 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 folliculin interacting protein 2
别名 KIAA1450, FNIPL, FNIP1 like protein, 241638F2
观测分子量125 kda
GenBank蛋白编号BC166693
基因名称 FNIP2
Gene ID (NCBI) 57600
RRIDAB_3671906
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Folliculin (FLCN)-interacting proteins 1 and 2 (FNIP1, FNIP2) are homologous binding partners of FLCN, a tumor suppressor for kidney cancer. FNIP2 was found to bind to the C terminus of FLCN and to AMPK, like FNIP1. FNIP2 competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PMID: 18403135).

实验方案

Product Specific Protocols
WB protocol for FNIP2 antibody 84371-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols