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验证数据展示

  • WB analysis of mouse heart using 11507-1-AP

    WB analysis of mouse heart using 11507-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11507-1-AP (FNTB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 11507-1-AP

    WB analysis of A431 using 11507-1-AP

    WB result of FNTB antibody (11507-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected A431 cells.

  • IP experiment of HeLa using 11507-1-AP

    IP experiment of HeLa using 11507-1-AP

    IP result of anti-FNTB (IP:11507-1-AP, 4ug; Detection:11507-1-AP 1:1000) with HeLa cells lysate 800ug.

  • Featured Product
  • KD/KO验证

FNTB Polyclonal antibody

FNTB Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11507-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

FNTB, FPTB, FTase beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, A431 cells, mouse skin tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11507-1-AP targets FNTB in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FNTB fusion protein Ag2061 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 farnesyltransferase, CAAX box, beta
别名 FNTB, FPTB, FTase beta
计算分子量 437 aa, 49 kDa
观测分子量 49 kDa, 44 kDa
GenBank蛋白编号BC020232
基因名称 FNTB
Gene ID (NCBI) 2342
RRIDAB_2106301
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49356
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.

实验方案

Product Specific Protocols
WB protocol for FNTB antibody 11507-1-APDownload protocol
IHC protocol for FNTB antibody 11507-1-APDownload protocol
IP protocol for FNTB antibody 11507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols