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c-Fos Monoclonal antibody

c-Fos Monoclonal Antibody for WB, ELISA
Cat No. 66590-1-Ig

产品说明书

CloneNo. 1G2C5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, ELISA and More (3)

FOS, Fos proto-oncogene, AP-1 transcription factor subunit, Cellular oncogene fos, C FOS, AP 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HSC-T6 cells, Jurkat cells, U-937 cells, RAW 264.7 cells, K-562 cells, THP-1 cells, NIH/3T3 cells

c-Fos is an extremely short-lived intracellular protein that needs to be detected in fresh samples to prevent its degradation.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66590-1-Ig targets c-Fos in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 c-Fos fusion protein Ag24340 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 FOS
别名 FOS, Fos proto-oncogene, AP-1 transcription factor subunit, Cellular oncogene fos, C FOS, AP 1
计算分子量 41 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC004490
基因名称 c-Fos
Gene ID (NCBI) 2353
RRIDAB_2881950
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP01100
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

c-Fos, also named as FOS and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. c-Fos is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, c-Fos undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the c-Fos protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the c-FOS protein becomes nuclear. c-Fos is a very short-lived intracellular protein, which is very easy to degrade. The calculated molecular weight of c-Fos is 40 kDa, but Phosphorylated c-Fos protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-Fos is an indirect marker of neuronal activity because c-Fos is often expressed when neurons fire action potentials. Upregulation of c-Fos mRNA in a neuron indicates recent activity.

实验方案

Product Specific Protocols
WB protocol for c-Fos antibody 66590-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Curr Biol

Neural processing of the reward value of pleasant odorants.

Authors - Maëllie Midroit
humanWB

Theranostics

KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.

Authors - Chengwan Zhang
  • KD Validated
mouse

Phytomedicine

Gastrodin alleviates NTG-induced migraine-like pain via inhibiting succinate/HIF-1α/TRPM2 signaling pathway in trigeminal ganglion

Authors - Chao Ma
mouse

Phytomedicine

Withaferin A protects against epilepsy by promoting LCN2-mediated astrocyte polarization to stopping neuronal ferroptosis

Authors - Zixian Zhou
humanIHC

J Transl Med

Integrating spatial transcriptomics and single-cell RNA-sequencing reveals the alterations in epithelial cells during nodular formation in benign prostatic hyperplasia

Authors - Xiawei Fei
mouseWB

J Headache Pain

SS-31 alleviated nociceptive responses and restored mitochondrial function in a headache mouse model via Sirt3/Pgc-1α positive feedback loop

Authors - Zhengming Shan
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