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  • Featured Product
  • KD/KO Validated

FOX2/RBM9 Monoclonal antibody, PBS Only

FOX2/RBM9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7G5

Cat no : 66976-1-PBS

Print datasheet

Synonyms

dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, FOX2/RBM9, fxh, HNRBP2, HRNBP2, RBFOX2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA

验证数据展示

  • WB analysis using 66976-1-Ig (same clone as 66976-1-PBS)

    WB analysis using 66976-1-Ig (same clone as 66976-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66976-1-Ig (same clone as 66976-1-PBS)

    WB analysis of HEK-293 using 66976-1-Ig (same clone as 66976-1-PBS)

    WB result of RBM9 antibody (66976-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM9 transfected HEK-293 cells. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66976-1-Ig (same clone as 66976-1-PBS)

    IHC staining of mouse brain using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOX2/RBM9 antibody included
  • IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)

    IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOX2/RBM9 antibody included
  • IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)

    IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOX2/RBM9 antibody included
Planning an IHC experiment? We recommend our IHCeasy FOX2/RBM9 Ready-To-Use IHC Kit. FOX2/RBM9 primary antibody included.

产品信息

66976-1-PBS targets FOX2/RBM9 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen FOX2/RBM9 fusion protein Ag28336 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RNA binding motif protein 9
Synonyms dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, FOX2/RBM9, fxh, HNRBP2, HRNBP2, RBFOX2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA
Calculated Molecular Weight 390 aa, 41 kDa
Observed Molecular Weight 48 kDa, 50 and 60 kDa
GenBank Accession NumberBC013115
Gene Symbol RBM9
Gene ID (NCBI) 23543
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43251
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)