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FOXC1 Polyclonal antibody

FOXC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30082-1-AP

Print datasheet

Synonyms

FREAC3, FREAC 3, Forkhead-related transcription factor 3, Forkhead-related protein FKHL7, FKHL7

验证数据展示

  • WB analysis using 30082-1-AP

    WB analysis using 30082-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30082-1-AP (FOXC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30082-1-AP targets FOXC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen FOXC1 fusion protein Ag32531 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box C1
Synonyms FREAC3, FREAC 3, Forkhead-related transcription factor 3, Forkhead-related protein FKHL7, FKHL7
Calculated Molecular Weight57kd
Observed Molecular Weight 70 kDa
GenBank Accession NumberNM_001453.3
Gene Symbol FOXC1
Gene ID (NCBI) 2296
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12948
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly. This antibody is specific to FOXC1. Phosphorylation modification of Foxc1 protein may be responsible for the larger molecular weight of detection compared with theoretical molecular weight (PMID:27708239;16403239).

实验方案

Product Specific Protocols
WB protocol for FOXC1 antibody 30082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols