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验证数据展示

  • WB analysis using 83476-6-RR (same clone as 83476-6-PBS)

    WB analysis using 83476-6-RR (same clone as 83476-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83476-6-RR (FOXC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83476-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83476-6-RR (same clone as 83476-6-PBS)

    FC experiment of HeLa using 83476-6-RR (same clone as 83476-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-6-RR, Clone:240403A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83476-6-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83476-6-RR (same clone as 83476-6-PBS)

    FC experiment of A549 using 83476-6-RR (same clone as 83476-6-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-6-RR, Clone:240403A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83476-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83476-6-RR

    Affinity and Kinetic Characterization of 83476-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83476-6-RR against Human FOXC2 were performed. The affinity constant is 0.328 nM.

FOXC2 Recombinant antibody, PBS Only

FOXC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83476-6-PBS

产品说明书

CloneNo. 240403A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

MFH 1, Mesenchyme fork head protein 1, Forkhead-related protein FKHL14, Forkhead box protein C2, FKHL14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83476-6-PBS targets FOXC2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 FOXC2 fusion protein Ag33121 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box C2 (MFH-1, mesenchyme forkhead 1)
别名 MFH 1, Mesenchyme fork head protein 1, Forkhead-related protein FKHL14, Forkhead box protein C2, FKHL14
计算分子量54kd
观测分子量 54-68 kDa
GenBank蛋白编号NM_005251
基因名称 FOXC2
Gene ID (NCBI) 2303
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ99958
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. (PMID: 8674414 9169153 19935708) This antibody recognizes the 56 kDa FOXC2 protein, but the phosphorylated FOXC2 may be a 56-65 kDa protein. The 62kDa band was also detected in the study(PMID: 19540201).